This website uses cookies to ensure you have the best experience. Learn more

Ap Biology Enzyme Synthesis Lab Report

1498 words - 6 pages

Enzyme Catalysis 牋牋牋牋Introduction and Overview- In this lab, we will observe the conversion of hydrogen peroxide (H2O2) into water and oxygen gas. An enzyme known as catalase facilitates this conversion reaction. The catalase enzyme acts as catalysis, helping lower the energy needed to activate the reaction while the enzyme itself is not affected. The reaction could take place without the help of catalase, but it would happen a lot more slowly because more energy is needed for the reaction.牋牋牋牋The substance being acted on by the enzyme is known as the substrate, and in this case is hydrogen peroxide. The enzyme changes these substrates ...view middle of the document...

5% H2O2 (Hydrogen Peroxide) 牋牋牋牋路 Catalase 牋牋牋牋路 1 ml syringe 牋牋牋牋 牋牋牋牋路 10 ml syringe 牋牋牋牋路 Stopwatch 牋牋牋牋路 Six 50 ml beakers 牋牋牋牋路 2 more beakers (any fairly large size) to store acid and hydrogen peroxide 牋牋牋牋Procedure- Part A: Demonstration of Presence of Catalase in Living Tissue.Note: Demonstration done by teacher. No procedure to be recorded, observations in Data section.牋牋牋牋Part B: Determination of Rate of an Enzyme Catalyzed Reaction.1. 牋牋牋牋Determine baseline- In this experiment, was figured by someone else. The baseline is the measure of a substance in a solution. In this experiment, the amount of H2O2 in a 5 ml solution was figured to be 3.2 ml. This baseline will be used for the duration of the experiment.2. 牋牋牋牋Prepare beakers- Extract 10 ml of H2O2 in syringe and dispense in beaker.3. 牋牋牋牋Start reaction- Extract 1 ml of catalase solution with syringe and dispense into beaker w/ H2O2. Swirl solution. Start stopwatch.4. 牋牋牋牋Stop reaction- After 10 seconds has elapsed, stop reaction by adding 10 ml of H2SO4, also known as sulfuric acid. This denatures the enzyme and reactions stop. The amount of remaining substrate can now be measured.5. 牋牋牋牋Measure amount of remaining H2O2- First, 5 ml of the solution must be extracted from the beaker w/ a syringe and placed into a clean beaker. The beaker is position underneath the burette and KMnO4 is slowly added to the solution until the solution no longer remains clear. The amount of KmnO4 used is calculated by finding the difference between the initial and final readings of the burette.6. 牋牋牋牋Repeat the experiment- Repeat steps 2 through 5 for 30, 60, 120, 180, and 360 seconds.Data Collected- Part A- After the H2O2 was added to a piece of potato, the potato appeared to release small bubbles.牋牋牋牋Part B- Below is a table of the data collected in this portion of the experiment: Table 1: Recorded Data of Part B Recorded and Calculated Information in Part B 牋牋牋牋 KmnO4 (ml) 牋牋牋牋 TIME (Seconds) 牋牋牋牋 牋牋牋牋10 牋牋牋牋30 牋牋牋牋60 牋牋牋牋120 牋牋牋牋180 牋牋牋牋360 牋牋牋牋 A. Baseline 牋牋牋牋3.2 牋牋牋牋3.2 牋牋牋牋3.2 牋牋牋牋3.2 牋牋牋牋3.2...

Other Essays On AP Biology Enzyme Synthesis Lab Report

The Effects of Smoking on Lung Tissue - Biology - Lab Report

2559 words - 11 pages slides of both normal lung and bronchogenic carcinoma were also observed and drawn to assist with the study. After completing the experiment, the mean beat frequency was calculated for each variable using both trials from each student in the lab. A bar graph was then created using these averages to display the data. The standard error was also calculated for each average and plotted onto each bar on the graph. ResultsD. Perklic The data graphed in

Lab report on lakes and health of rivers - AP environmental - lab report

584 words - 3 pages Beau Jeffries Mrs. Whitehead AP Environmental Science 27 April 2018 Water Quality of the Rose River Purpose: Our purpose is to collect​ ​and analyze the physical and chemical characteristics of the river to determine the quality of the water. Hypothesis/ Background: Based on the first impressions of the stream we hypothesized that the stream is healthy and the surrounding areas are healthy. Materials: - Orange - Meter Stick - Measuring Tape

Effect on Solute Concentration in Dialysis Tubing After 30 Minutes - AP Biology - Lab Repott

1662 words - 7 pages , and additional help from my teacher. Previous to the lab, my biology class had recently covered diffusion, and how it functions within the cell. We first covered basic diffusion: the movement of a substance from high concentrations to low concentrations. Then we moved onto the cell semipermeable membrane is what dictates which substances pass through. Following that, we covered osmosis: the movement of a solvent into a higher concentration in

Heat of Combustion of Solid Magnesium using Hydrochloric Acid and Magnesium Oxide in a calorimeter. - AP Chemistry - Lab report

634 words - 3 pages ) Mg (s) + 2 HCl(aq) → MgCl​2​(aq) + H​2​ (g) (3) 2H​2​(g) + O​2​(g) → 2H​2​O(l) ΔH for this reaction ​-571.6 kJ. In this experiment heats of reaction for equations (1) and (2) will be determined. Pre-lab exercise In the space provided below, “​rewrite”​ and combine equations (1), (2), and (3) to obtain the desired target equation. (1) ​MgCl​2​(aq) + H​2​O(l) → MgO(s) + 2 HCl(aq) (2) ​Mg (s) + 2 HCl(aq) → MgCl​2​(aq) + H​2​ (g) (3) ​H​2​(g

Raven Chapter 4 Guided Notes: Cell Structure - AP Biology - Guided Notes

662 words - 3 pages , involved in lipid synthesis, membrane formation and detoxification JHalloran Text Box contains bound ribosomes, synthesis of membrane bound and secretory proteins JHalloran Text Box "post office" receives , repackages and modifies outgoing material from ER and incoming material from cell membrane Name _____________________________ AP Biology 3 of 4 Lysosome Vacuole Mitochondria Chloroplast Peroxisome Cytoskeleton Microtubule Actin Microfilament

DNA

1661 words - 7 pages correct amino acids to the mRNA so they can be aligned in the right order by the ribosomes. (Heath,116) For protein synthesis to begin, the two parts of a ribosome must secure itself to a mRNA molecule.(Miller, 151)Methods and Materials:For the first part of the lab, colored paper clips were needed to construct two DNA strands. Each color paperclip represented one of the four nitrogenous bases. Black was used as adenine, white was thymine, blue

biology the longer the catlase the better the reaction - 9t sydney girls highschool - research paer

1126 words - 5 pages Biology assessment task 1 Catalase Enzyme Reaction Ariba Afsheen Background information All organisms rely on enzymes to catalyze chemical reactions. An enzyme is a biological catalyst that increases the rate of chemical reaction by lowering the level of activation energy necessary to start the reaction. Without enzymes, many of the chemical reactions that occur within living things would proceed to slowly to be useful. Enzymes speed up these

The dynamic cell.. ....... .... ... ... - south university online / bio1020 - essay on cells

922 words - 4 pages have the ability to reproduce using binary vision, this is a form of asexual reproduction, although it is more common in prokaryotic cells, some eukaryotes, such as amoeba can also have this ability. Golgi apparatus takes proteins and processes and packages them. The Golgi apparatus is made up of cisternae sacs. These sacs have different functions as well as different regions. Each region has a specific enzyme to help with the designated task to

Colloidal synthesis of gold nanoparticles - city university of hong kong - coursework

1665 words - 7 pages City University of Hong Kong Department of Applied Physics and Material Science AP 4178 Nanostructure and Nanotechnology Lab Report of Lab 1. Colloidal synthesis of gold nanoparticles Date of Exp.: 14/10/2015 Liu Tsz Kwan Objective When we complete this laboratory, we are able to: 1. Applicate the concept of light scattering on particles in colloid suspension. 2. Differentiate the difference between reflection and absorption of UV. 3. Applicate

Biosynthesis of antibiotics drug Daptomycin - Microbiology/postgraduate - Essay

1488 words - 6 pages . Researchers have indicated that the genes in S. Roseosporus that are responsible for the formation of the lipoprotein antibiotic, acylate the N-terminal amino acid of Daptomycin. The initiation of translation of this lipopeptide chain involves an activator of the fatty acid, which is the acyl-CoA ligase. Additionally an acyl carrier protein or ACP is also, to which the fatty acid remains attached. The N-terminal end of the C domain of the NRPS enzyme

Chemoselective of Aromatic Compounds Lab Report - organic chemistry - Lab Report

1624 words - 7 pages Casto 1 Morgan Casto Dr. Hillwig Chemoselective, Syntheses of Natural Fragrance and Perfume Additives Lab Report 2/26/19 Introduction: One of the most important concepts within chemistry is the chemoselectivity of reactions. This concept deals with a how reagent will choose a functional group to react with, resulting in the formation of the major product. Our lab studied 6 reactions: three oxidation and three reduction of the three compounds

Similar Papers

Effect Of Temperature On Enzyme Activity Ap Biology Research Lab

898 words - 4 pages The Effect of Temperature on Enzyme Activity The purpose of this lab practice is to investigate the effects of temperature on the denaturation of enzymes. Introduction: The main biological concept explored was enzyme activity, enzymes are biological molecules (proteins) that act as catalysts and help complex reactions occur everywhere in life. Enzymes are impacted by temperature and pH of their environment, because these factors can denature an

Effect On Solute Concentration In Dialysis Tubing After 30 Minutes Ap Biology Lab Report

1662 words - 7 pages , and additional help from my teacher. Previous to the lab, my biology class had recently covered diffusion, and how it functions within the cell. We first covered basic diffusion: the movement of a substance from high concentrations to low concentrations. Then we moved onto the cell semipermeable membrane is what dictates which substances pass through. Following that, we covered osmosis: the movement of a solvent into a higher concentration in

Report About Biology Lab Birds Lab Lab Lab Lab Lab Alibi Alandj Hdsjkhd Bdsvkfb Bfdskbfks Biology Biology

1424 words - 6 pages Natural Disasters Risk Report for Address, City, State ZIP INTRODUCTION Lorem ipsum dolor sit amet, consectetuer adipiscing elit, sed diam nonummy nibh euismod tincidunt ut laoreet dolore magna aliquam erat volutpat. Ut wisi enim ad minim veniam, quis nostrud exerci tation ullamcorper suscipit lobortis nisl ut aliquip ex ea commodo consequat. Duis autem vel eum iriure dolor in hendrerit in vulputate velit esse molestie consequat, vel illum

Factors That Affect Plant Growth Grade 11 Biology Lab Report

1573 words - 7 pages Factors that Affect Plant Growth lab Tegan Misuraca Purpose: This experiment was completed to show how light and nutrients affect plant growth. Theory​: Plants require sunlight because they have the ability to convert heat energy from sunlight into a usable energy, using photosynthesis. Without sunlight this process would not be able to occur and after a certain point the plant wouldn’t release oxygen and would die.Plant need sufficient amount