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Ap Biology Enzyme Synthesis Lab Report

1498 words - 6 pages

Enzyme Catalysis 牋牋牋牋Introduction and Overview- In this lab, we will observe the conversion of hydrogen peroxide (H2O2) into water and oxygen gas. An enzyme known as catalase facilitates this conversion reaction. The catalase enzyme acts as catalysis, helping lower the energy needed to activate the reaction while the enzyme itself is not affected. The reaction could take place without the help of catalase, but it would happen a lot more slowly because more energy is needed for the reaction.牋牋牋牋The substance being acted on by the enzyme is known as the substrate, and in this case is hydrogen peroxide. The enzyme changes these substrates ...view middle of the document...

5% H2O2 (Hydrogen Peroxide) 牋牋牋牋路 Catalase 牋牋牋牋路 1 ml syringe 牋牋牋牋 牋牋牋牋路 10 ml syringe 牋牋牋牋路 Stopwatch 牋牋牋牋路 Six 50 ml beakers 牋牋牋牋路 2 more beakers (any fairly large size) to store acid and hydrogen peroxide 牋牋牋牋Procedure- Part A: Demonstration of Presence of Catalase in Living Tissue.Note: Demonstration done by teacher. No procedure to be recorded, observations in Data section.牋牋牋牋Part B: Determination of Rate of an Enzyme Catalyzed Reaction.1. 牋牋牋牋Determine baseline- In this experiment, was figured by someone else. The baseline is the measure of a substance in a solution. In this experiment, the amount of H2O2 in a 5 ml solution was figured to be 3.2 ml. This baseline will be used for the duration of the experiment.2. 牋牋牋牋Prepare beakers- Extract 10 ml of H2O2 in syringe and dispense in beaker.3. 牋牋牋牋Start reaction- Extract 1 ml of catalase solution with syringe and dispense into beaker w/ H2O2. Swirl solution. Start stopwatch.4. 牋牋牋牋Stop reaction- After 10 seconds has elapsed, stop reaction by adding 10 ml of H2SO4, also known as sulfuric acid. This denatures the enzyme and reactions stop. The amount of remaining substrate can now be measured.5. 牋牋牋牋Measure amount of remaining H2O2- First, 5 ml of the solution must be extracted from the beaker w/ a syringe and placed into a clean beaker. The beaker is position underneath the burette and KMnO4 is slowly added to the solution until the solution no longer remains clear. The amount of KmnO4 used is calculated by finding the difference between the initial and final readings of the burette.6. 牋牋牋牋Repeat the experiment- Repeat steps 2 through 5 for 30, 60, 120, 180, and 360 seconds.Data Collected- Part A- After the H2O2 was added to a piece of potato, the potato appeared to release small bubbles.牋牋牋牋Part B- Below is a table of the data collected in this portion of the experiment: Table 1: Recorded Data of Part B Recorded and Calculated Information in Part B 牋牋牋牋 KmnO4 (ml) 牋牋牋牋 TIME (Seconds) 牋牋牋牋 牋牋牋牋10 牋牋牋牋30 牋牋牋牋60 牋牋牋牋120 牋牋牋牋180 牋牋牋牋360 牋牋牋牋 A. Baseline 牋牋牋牋3.2 牋牋牋牋3.2 牋牋牋牋3.2 牋牋牋牋3.2 牋牋牋牋3.2...

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