Cure Lab Reflection: Oral Microbiome University Of Minnesota/Biol1009 Research Paper

1001 words - 5 pages

Oral Microbiome
Question: Which sugar (artificial or natural) is going to lower the pH to prevent streptococcus mutans and lactobacilli?
Streptococcus mutans has been identified as an important agent in dental caries. In the microbiome, bacteria interact with each other as they attempt to establish themselves in this environment. (Kuramitsu, 2005). A dynamic relation between sugars and dental caries exists. Sugars and other carbohydrates, after being hydrolyzed by salivary amylase, provide substrate for oral bacteria, which in turn lowers plaque and salivary pH. The result of this action is the beginning of tooth demineralization. Dietary factors such as sugarless chewing gum—especially those containing xylitol, and consumption of sugars in meals as opposed to in between meals may reduce the risk of caries. Low pH in the microbiome favors the growth of the acidogenic bacteria, such as streptococcus mutans. In contrast, a diet low in added sugars and carbohydrates and high in calcium may favor remineralization (Touger-Decker, 2003).
Hypothesis: Comparing artificial sugars to natural sugars, natural sugars will result in the increase of pH, which will decrease Streptococcus mutans and Lactobacilli.
Prediction: Natural sugars will increase the pH, which will lead to an increase in zone of inhibition. The higher the pH, the larger the zone of inhibition.
Experimental Design:
1. Take two petri dishes, one for streptococcus mutans and the other for lactobacilli.
2. Label the dishes (one for streptococcus mutans and the other for lactobacilli.)
3. Plate 200ul of specified bacteria to each of the dishes.
4. Spread the bacteria equally throughout the dish with a T shaped spreader
5. Steralize t
6. Create a positive control disk with bleach and add this to both dishes
7. Create a negative control disk with water and add this to both dishes
8. Measure 4 Tbsp of deionized water in a beaker set to room temperature.
9. Mix 1tbps of cane sugar with the warmed water. The natural and artificial sugars are the independent variables.
10. Submerge two disks in the mixture for 30 seconds
11. Add one disk to each petri dish
12. Repeat steps 5-8 with honey and aspartame
13. Seal petri dishes with paraffin wax
14. Incubate at 37 degrees for one week
15. Measure the diameter of the zone inhibition. The zone of inhibition is the dependent variable.
16. Repeat for a total of 4 trials
Data:
Average Zone of Inhibition in Lactobacilli and Streptococcus Mutans
Week 1
Week 2
Week 3
Week 4
Red Plate
White Plate
Red Plate
White Plate
Red Plate
White Plate
Red Plate
White Plate
Cane
Sugar
0
0
0
0
0.333
0
0
0
Aspartame
2.4
1.86
2.66
2.3
1.76
1.86
1.64
1.56
Honey
0
0
0
0
0
0
0
0
Bleach
0
0
0
0
0
0
0
0
Water
0
0
0
0
0
0
0
0
Std.
dev
0.96
0.744
1.064
0.92
0.68298744
0.744
0.656
0.624
SEM
0.42932505
0.33272692
0.47583527
0.41143651
0.30544127
0.33272692
0.29337212
0.27906128
+/-
2SEM
0.8414771
0.65214475
0.93263712
0.80641556
0.59866488
0.65214475
0.57500935
0.54696012
Conclusions:...

Other Essays On cure lab reflection: oral microbiome - university of minnesota/biol1009 - research paper

Effect of Temperature on Enzyme Activity - AP biology - Research Lab

898 words - 4 pages The Effect of Temperature on Enzyme Activity The purpose of this lab practice is to investigate the effects of temperature on the denaturation of enzymes. Introduction: The main biological concept explored was enzyme activity, enzymes are biological molecules (proteins) that act as catalysts and help complex reactions occur everywhere in life. Enzymes are impacted by temperature and pH of their environment, because these factors can denature an

Experiment 38 Pre - Lab notes and agenda - University of Texas Rio Grande Valley - Pre-Lab explanation on the process.

537 words - 3 pages test ➢ Moisten a red litmus paper with water, add 1-2 mL of 6M NaOH to solution and suspend litmus above the solution. Litmus paper changing from red to blue confirms presence of NH4+ Part D – Fume Hood Reducing Copper II ion ➢ 5 mL in a 150 mm test tube, polish 1 cm Zn strip (with steel wool) and place it into the solution. Let stand for 10-20 minutes. Decant solution and save for part E.1 DO NOT THROW AWAY In our case, remove solution and leave

critical reflection of a personal academic journey using the MBTI - University of Gloucestershire - Essay

3345 words - 14 pages . Upon reflection I think the next time I research into reflective practice, I will not have any distractions surrounding me and out 100% of my attention towards the task. Upon further reflection, having read back through my account, I think I need to put more depth into my writing. As this is my first reflective account I believe that I need more practice and research, this will help me to recognise my strengths and weaknesses which will enable

sheep heart lab report for fhhs science class - forest hills high school/ science - research paper

631 words - 3 pages Ralph Marinas Mr. Cifuentes Anatomy and Physiology Pd. 8 April 9, 2018 Sheep Heart Dissection Lab Report Objective: To observe the external and internal structure of a sheep heart and compare it to a human heart. Materials: 1. 1 triple beam balance 2. 1 safety goggle 3. 2 pairs of gloves 4. 1 lab apron 5. 1 mask 6. 1 tray 7. 1 sheep heart 8. Paper towels 9. 1 scalpel 10. 1 scissor 11. 1 ruler 12. 1 pencil 13. Paper 14. 1 camera Procedure: 1

The Antecedents, Facilitators and Consequences of Parasocial Relationships - University of Toronto/PSY424 - Research Paper

3941 words - 16 pages ; secondly, what processes and media use over time would facilitate PSR between media user and media figures; thirdly, how PSI might, as its originators put it, be “integrated into the matrix of usual social activity” (Horton & Wohl, 1956) and what impact would it bring. This paper attempts to answer the above questions by reviewing existing literatures and give suggestions for future research directions on the topic. Literature Review 1.1

Augmented Reality Research Paper - West Chester University, IST - Research Paper

1034 words - 5 pages Augmented Reality and Video Games Augmented Reality and Video Games Abstract This paper is about views in Augmented Reality and video games. The advancements of entertainment and types of games with Augmented Reality are presented. These topics are then analyzed by the technology usage, and genre. Augmented Reality is an interactive experience of a real-world environment where the objects that reside in the real-world are "augmented" by computer

Analysis on The Pattern of Mythical Heroes' Deaths - University of California, Los Angeles - Research Paper

2100 words - 9 pages Page �1 Name: Luming (Max) Zhou GE30 Section 1F GSI: Hannah Byers-Brown Paper #2 Date: March 11th, 2019 Beauty of Heroic Death Throughout time, people have created heroes that we can always remember at the tip of our tongues, either they came from novels, comic books, movies, or ancient myths and legends. For most of the heroes, they can be categorised into two general types: ones that live happily ever after the “impossible” quest and ones that

socail media effects on romantic relationships - University Of South Alabama, fundimentals of communication research - Reseach paper

1815 words - 8 pages , solidarity, transparency, cheating etc. In this paper, the role that social media plays on romantic relationships is investigated. This study is hypothesized that social media can have negative and positive impacts on romantic relationships. The following literature reviews have attempted to demonstrate and support this hypothesis. Cizmeci, E. states the use of a mobile device can sum up to be a good thing. When trying to maintain a

Research Paper Database Management Systems - MIS Howard University - Research Paper

3134 words - 13 pages 1 DATABASE MANAGEMENT SYSTEMS (DBMS) Database Management Systems (DBMS) How they affect our everyday life. Xavier Edwards Howard University Management Information Systems 001-01 Dr. Agarwal October 31, 2018 Abstract A database is a structured set of data held in computer or software, especially one that is accessible in various ways. Databases have been in existence since the early 1960s; however, the most universally used type of database was

African American Reading Reflection - University of Iowa, Culturally Competent Nursing - Book Report

905 words - 4 pages African American Reading Reflection Aundria Wedig Trinity College of Nursing and Health Sciences 1. What is the main point? The African American patient will most likely speak a “Black English” language, generally embrace a close sense of personal space, and perceive time as flexible and elastic. These important African American cultural aspects are of vital attention to the nurse when providing care to ensure culturally competent and harmless

Performing Vocal Music of the Renaissance - University of Mississippi - Introduction to Music Research (Graduate Level) - Research Paper

2309 words - 10 pages Culpepper 2 Richard Culpepper 8 December 2016 Dr. Thomas Peattie Final Research Paper Performing Vocal Music of the Renaissance Music has existed for thousands of years, if not longer. The earliest musical notation known to date is from a cuneiform tablet created ca. 2000 BC, found in modern day Iraq in the 1960s.[footnoteRef:1] The oldest known musical instruments are even older than that. At an age of ca. 40,000 years old, four carved-bone

Similar Papers

Personal Reflection On Group Assignment University Of Charles Sturt Personal Reflection

1152 words - 5 pages The purpose of this paper is to reflect my personal experience working part of a group in Assignment 2 using the concepts and theories from Topic 6 of Groups and Teams. My reflection has grasped my ability to learn and recommend strategies to manage performance of the team. Through the brief background introduction of the students, this gave me an insight of each person and the groups. I joined Group Report 01 because I felt they were the most

Reflection About The Consumer Behaviour University Of Liverpool/ Marketing Assignment

1214 words - 5 pages Module Code: ULMS 795 Module Leader: Matthews Paul Module Code:ULMS 795 Module Title: Consumer Behaviour Tutor Name: Matthews Paul Title: The reflection about the differences in traditional customs between China and Britain Student Name: Lishan Luo (201281240) Date of Submission: 16/03/2018 Word count: 958 Module Code: ULMS 795 Module Leader: Matthews Paul 1 In order to satisfy the needs and desires, there is a process for individuals or groups

The Economics Of University Accessibility Ap Seminar Research Paper

1380 words - 6 pages Rahman who are research students from MABE, took data from the Canadian Labour Force Survey and discovered that higher tuition levels in the 1990s did in fact reduce the probability of university participation of persons aged 17-19 (Johnson, 2005). Furthermore, university applications are getting more and more competitive; which minimizes the possibility of acceptance and scholarship opportunity. Our main question is, are students restricted to

Arabic Architecture And Influence University Of Colorado Boulder Research Paper

2482 words - 10 pages no doubt that it influenced early modern Europe and, therefore has relevance in today’s world. Specifically, European art and architecture borrow heavily from the Islamic world. As seen in this paper, the pointed, four-centered, and horseshoe arch that are evident in most of the ancient buildings in Europe borrow heavily from Islamic architecture. The same can be said about domes. In addition, music, decorative art, ornament style and carpets all