Enzyme Action Essay

1557 words - 7 pages

1.Problem / PurposeThe purpose of this experiment is to investigate the properties of an enzyme - substrate reaction2.HypothesisHydrogen peroxide is a very reactive compound that can be used for a variety of reactions including bleaching and disinfecting minor wounds. Acting as an oxidizing agent, it is also toxic to cells, hence its value as a disinfecting agent that disrupts the metabolism of bacteria. Our body cells and those of many other animals contain an enzyme called catalase that accelerates the conversion of toxic hydrogen peroxide to water and oxygen gas.In this experiment, if the concentration of the enzyme is increased, then the reaction will proceed faster and if the ...view middle of the document...

03 * 25 g = 0.75 g64.08 g Hydrogen Peroxide----------- 22400 g Oxygen Gas0.75 g Hydrogen Peroxide ------ x g Oxygen gasx = (1.3)x = 246.7685 g Oxygen GasTheoretically, 246.7685 mL of Oxygen Gas should be released6.3.Question #3:a) The results of the experiment comparing the amount of oxygen evolved as time elapses are presented in the graph below.b) The results of the experiment comparing the amount of oxygen evolved after 20 seconds, for each enzyme concentration used are presented in the graphs below.6.4.Question #4: The results of other members of the class are presented in comparison with our results in the observation table. The results for the amount of oxygen evolved at the end of the experiment are within a 0 - 5 mL range.The sources of error are grouped into:Human errorsAll these errors are due to the way each member of the class reads the instruments, how they appreciate and approximate in + or - when the reading is between two divisions on the reading on the graduated cylinder. For example, if the water level in the graduated cylinder is between 240 and 250 mL, one group might approximate 244 and another might approximate 246.Apparatus and instrumental errorsDifferent members of the class used different instruments (ex: syringe, clock, graduated cylinder, hose) substances such as hydrogen peroxide obtained from different bottles with different concentrations, all these producing different results within all members of the class. For example, if the concentration of Hydrogen Peroxide is 3% (as theoretically given) then the amount of oxygen evolved should be 246.77 mL, but because each group extracted from different bottles, some might be 3.05% (giving 288mL) while others 2.99 % (giving 240 mL).Also, the syringe may not be calibrated for the temperature in the lab.6.5.Question #5: The amount of oxygen produced in our experiment and the theoretical yield are slightly different. In addition to the errors presented in paragraph 6.4., there could be many little difference between the real values involved in the experiment and the values that were considered in the theoretical approach, for example, the hydrogen peroxide concentration used in the experiment could be slightly different than the theoretically assumed value -> 3.0%. This would produce different amounts of oxygen.The volume of air is measured at a pressure bigger than the standard atmospheric pressure because of the immersion of the tube in water. The pressure in the tube, when it is full of gas, is equal to the pressure at the bottom of the tube that is the atmospheric pressure plus the pressure due to the depth of immersion, so the gas contained in the tube is slightly compressed giving a measured volume that is smaller than the correct volume of gas at atmospheric pressure.6.6.Question #6: Enzymes are globular proteins whose shapes are specialized to perform temporary associations with the molecules that are reacting. By putting stress on particular chemical bonds,...

Other Essays On Enzyme Action

Niemann-Pick Disease Essay

1272 words - 6 pages Niemann-Pick disease isn't actually one disease at all; it actually is a term used to represent a group of diseases which affect the metabolism. Each member of the group is a direct result of genetic mutations. Of the group, the three most common and most recognized forms of are Types A, B, and C.Types A and B or Niemann-Pick disease are both causes by the insufficiency of the enzyme acid sphingomyelimase, also known as ASM. Sphingomyelimase is

Biosynthesis of antibiotics drug Daptomycin - Microbiology/postgraduate - Essay

1488 words - 6 pages ring of Daptomycin. However, other studies have shown that dptI is not directly involved in the methylation of glutamate, but is involved in an indirect pathway. The substrate for DptI enzyme is α keto glutarate, which on action of DptI is converted to 3-methyl-2-oxoglutarate. This intermediate is further transaminated to yield methyl glutamate. Research using Streptomyces coelicolor have demonstrated that an aminotransferase catalyses the

An Essay About Inherited Diseases. It Gives Examples And Explains Them Very Well

4787 words - 20 pages the best of care, children with the disease die in early childhood, usually by the age of five. An estimated 20 cases of TSD are diagnosed in the United States each year.TSD is caused by a defective gene that is unable to produce the enzyme hexosaminidase A (hex A). This enzyme is necessary to break down GM2 ganglioside, a fatty substance in the nerve cells of the brain. Children with TSD have no hex A; as a result, GM2 ganglioside builds up in

Revolution of DNA In Class Lecture Notes - University of Virginia - Class notes

1849 words - 8 pages utilitarian procedure for discerning the morally right course of action is theoretically simple: determine which action _____. MAXIMIZES WELL-BEING · Deoxyribose differs from Ribose in that… IS ONE OF THE BUILDING BLOCKS OF DNA DNA REPLICATION · Polymerase: enzyme that makes new DNA from old DNA · Double helix is coiled. The helix needs to be unwound. Topoisomerase relaxes the coil and Helicase unzips the DNA. The nucleotides are now exposed. DNA

Pharmacology Exam 4 Study Guide- The Cardiovascular System (20) - Fairleigh Dickinson University - Study Guide

3138 words - 13 pages The Cardiovascular System (20): ● Drug therapy hypertension: o RAAS Suppressants (4) Hypertension (HTN), Heart Failure, Diabetic Nephropathy, left ventricular dysfunction following myocardial infarction (MI) ▪ ACE inhibitors- ​captopril (Capoten) ● Expected Pharmacologic Action: Blocks production of angiotensin II, Vasodilation (arteriolar), Urinary excretion of sodium and water, Urinary retention of potassium ● Side and Adverse Effects: o

Cloning

2250 words - 9 pages new cells,' Eastman said. 'By using telomerase, we can take a normal cell and convert it into one having an infinite capacity to regenerate. By adding the cloning technology used to create Dolly, we can eliminate the threat of rejection.' Telomerase is an enzyme that causes a cell to become "immortal" by removing normal limits to the number of times it can reproduce. Geron owns or co-owns patents with claims on two distinct molecules that

Childhood disorders Chapter 1-5 - Brooklyn College/ Psychology 3240 - Notes

3355 words - 14 pages Adolescent Depression Medication PASCET-Primary and Secondary Control Enhancement Training ACTION- The ACTION program CWDA- Adolescent Coping with Depression Program BP: (Bipolar Disorder) Criteria: *BP1 *BP2 *Cyclothymic *NOS Prevalence: *In samples of youth 7-21, .5%- 2.5% worldwide *Rapid cycling can be common- 4 episodes over a 1 year period) in 80% of children. *most common= bipolar 2, cyclothymic, other (rather than BP1) Comorbidity: *anxiety

Character In "The Yellow Wallpaper" By Charlotte Perkins Gilman

1587 words - 7 pages The short story 'The Yellow Wall-Paper' written by Charlotte Perkins Gilman is a cry for freedom. This story is about a woman who fights for her right to express what she feels, and fights for her right to do what she wants to do. The narrator in this short story is a woman whose husband loves her very much, but oppresses her to the point where she cannot take it anymore. This story revolves around the main character, her oppressed life, and her

Edgar Allen Poe's View Of Death In "The Fall Of The House Of Usher"

1836 words - 8 pages Edgar Allen Poe's Symbolism of Death in 'The Fall of the House of Usher'Death is defined as, 'The termination or extinction of something' (American Heritage Dictionary). Edgar Allen Poe uses this description in 'The Fall of the House of Usher' in different ways. Poe's intention when writing 'The Fall of the House of Usher' was not to present a moral, lesson, or truth to the reader; he was simply trying to bring forth a sense of terror to the

"Slaughterhouse Five" By Vonnegut

2264 words - 10 pages exists because it eliminates the 'Why me?' question. Guilt is a feeling of responsibility or remorse for some offense, crime wrong ;a feeling of culpability. For example if one steals a hundred dollars, one would feel remorse over that action and wish one had not done it. Under the Tralfamadorian outlook Billy Pilgrim does not have to feel remorse for being saved because that is how it was and always will happen. He does not have to feel guilt or

Psychoanalysis And Freud

2420 words - 10 pages Psychoanalysis is a system of psychology originated by the Viennese physician Sigmund FREUD in the 1890's and then further developed by himself, his students, and other followers. It consists of three kinds of related activities: (1) a method for research into the human mind, especially inner experiences such as thoughts, feelings, emotions, fantasies, and dreams; (2) a systematic accumulation of a body of knowledge about the mind; and (3) a

Similar Papers

Biology The Longer The Catlase The Better The Reaction 9t Sydney Girls Highschool Research Paer

1126 words - 5 pages cells which is produced as a result of cellular respiration, and prevent cell death, by not allowing the chemical to accumulate within the body. This investigation models the action of enzymes in cells where hydrogen peroxide is the substrate and catalase the enzyme representative of the lock and key model. Aim The aim of this experiment is to investigate the correlation between temperature of hydrochloric acid and the levels of catalase enzymic

What Enzymnes Are? Essay

2561 words - 11 pages EnzymesIn this essay, the different types of enzymes existing are going to be discussed and how they are used in industry is also going to be discussed. There are 2000 different enzymes existing in the world. These enzymes are grouped according to the type of reaction they catalyze, (speed up). There are a thousand different reactions taking place in an individual cell and each reaction involves a specific enzyme. There are six major

Chemical Digestion Vs Mechanical Digestion Lower Merion High School/Honors Anatomy Assignment

436 words - 2 pages , where no significant digestion of carbohydrates takes place. The bolus then enters the stomach, where the acidic environment stops the action of the amylase enzyme. The next step of carbohydrate digestion takes place in the duodenum. It mixes with the digestive secretion from the pancreas, liver, and gallbladder, which breaks down the food into monosaccharides (glucose). The monosaccharides are produced and absorbed by the small intestine and then

A Literature Review Based On The Decounting Kmart Australia Robina Essay

552 words - 3 pages ). Its specific function, however, varies with plant variety. Barley (Hordeum vulgare) is a widely studied monocotyledon and an important agricultural crop. GA increases germination rates in barley seeds by increasing the conversion of complex carbohydrates to simple, useable sugars. The way in which GA promotes germination in barley can be considered a model for the action of GA in all monocotyledons (Blackton, 2008). As with most seeds, the barley