Identifying Pigments In Spinach And Red Leaves Using Chromatography Biology Uni Grade 12 Biology Formal Lab

1487 words - 6 pages

Identifying pigments in spinach and red leaves using chromatography
Inside----. Purpose of this lab was to identify the pigments in spinach and red leaves that make up their color. To do this an extract from the plants is added to a strip of chromatography paper and put into a solvent (10% acetone, 90% petroleum ether). The solvent gets sucked up and draws the extract with it. This action leaves different colored marks on the paper strip. Pigments found in spinach and red leaves has different solubility. Using Rf value we can identify each pigment. Errors that may occur is spill of plant extract on the chromatography paper when using a pipette, fine capillary tubes is recommended.
Chloroplasts, small, green, well-defined bodies (plastids) in the protoplasts of the cells in many photosynthetic organisms. (Cooper, 2000) The color is due to chlorophyll content, but they also contain yellow, orange or red carotenoids. Inside the chloroplast there is a network of membranes, tylakoid membranes, which contain pigments (chlorophyll a and b and carotenoids (carotene and xanthophyll)), as well as electron and prototransmitters that participate in photosynthesis light reaction. These are organized into two photosystems, photosystem II, where the water cleavage occurs, and oxygen is excreted, as well as photosystems in which NADPH2 is formed. Chromatography is exploited that the substances are of different solubility. Leaving an organic solvent pull up in a paper, the compounds that are readily soluble in the solvent used is coated with this, while the substances that are less soluble will be the same location in the paper or only a moving a bit. A bit of the sample to be analyzed is placed on chromatography paper, which is then immersed in a solvent. The solvent would then be sucked up, pass the stain and the solute drag with them, depending on where the substance is soluble in the solvent (Woodford, 2009).
The purpose of this lab was to separate compounds in spinach to see what pigments there is in spinach. If chromatography paper with spinach extract on it is put into a solution of petroleum ether, then we can see different pigments appearing on the paper. The prediction here is that spinach will have green and yellow pigments.
The purpose of the other lab where we used red leaves instead of spinach is to see what pigments red leaves has. If red leaf extract on a chromatography paper it is put into a solution of petroleum ether, then we can see different pigments appearing on the paper. The prediction here is that red leaves have red and yellow pigments.
Observation and result
When spinach extract was added to the chromatography paper and dipped down in the petroleum ether solution we could see that the paper sucked the solvent (10% acetone-90%petroleum ether) up. The solution got soaked up by the paper and when it reached the spinach extract it transported it upwards. After about 5 minutes the paper was soaked all the way up and...


Factors that affect plant growth - grade 11 biology - lab report

1573 words - 7 pages Factors that Affect Plant Growth lab Tegan Misuraca Purpose: This experiment was completed to show how light and nutrients affect plant growth. Theory​: Plants require sunlight because they have the ability to convert heat energy from sunlight into a usable energy, using photosynthesis. Without sunlight this process would not be able to occur and after a certain point the plant wouldn’t release oxygen and would die.Plant need sufficient amount

report about biology lab birds lab lab lab lab lab alibi alandj hdsjkhd bdsvkfb bfdskbfks - biology - biology

1424 words - 6 pages the Red Cross Ready ( site goes here. Conclusion Lorem ipsum dolor sit amet, consectetuer adipiscing elit, sed diam nonummy nibh euismod tincidunt ut laoreet dolore magna aliquam erat volutpat. Ut wisi enim ad minim veniam, quis nostrud exerci tation ullamcorper suscipit lobortis nisl ut aliquip ex ea commodo consequat. Duis autem vel eum iriure dolor in hendrerit in vulputate velit esse molestie consequat, vel illum dolore eu

Practical about beetroot pigments leaking - alevel biology - Biology practical write up

1447 words - 6 pages tubes and placed in a room temperature water bath. · After this, we cut 12 discs of beetroot using a ruler measuring 1mm in thickness ensuring all beetroot cylinders are of uniform thickness. · We then used the strainer to rinse the beetroot discs under the tap each piece to remove the excess red dye that leaked during the cutting procedure. · Then we added two pieces of the 1mm beetroot cylinders to each of the 6 test tubes containing the different

Yeast Genetics Biology Lab FlowChart - Intro Biology Laboratory - Biology Lab Procedural Flow Chart

831 words - 4 pages not. 12. Record the complementation results, using a + to indicate growth and – to indicate no growth. Seeing which strains mated to create diploids that can vs. cannot grow with and without adenine will help us deduce what the complementation pattern is between the three strains of type A yeast and the three strains of type Alpha yeast. 13. Analyze the replica plating results to deduce complementation patterns amongst the different strains

AP Biology Enzyme Synthesis Lab Report

1498 words - 6 pages Enzyme Catalysis 牋牋牋牋Introduction and Overview- In this lab, we will observe the conversion of hydrogen peroxide (H2O2) into water and oxygen gas. An enzyme known as catalase facilitates this conversion reaction. The catalase enzyme acts as catalysis, helping lower the energy needed to activate the reaction while the enzyme itself is not affected. The reaction could take place without the help of catalase, but it

The Impact of Red Bull and Berocca on Urine Content - BSDE, Biology - Research Paper

3407 words - 14 pages another 2 times 11. Steps 4-9 were repeated using berocca 4.0 Results Figure 1 Dipstick Results Pre and Post Red Bull Consumption The values of these colours can be found in the appendix. However, a table and graphical representation of these values can be seen below. Pre-Red Bull 2-3 Hours After 24 Hours After pH LEU NIT KET BIL PRO GLU UBG pH LEU Hb NIT KET BIL PRO GLU UBG pH LEU Hb NIT KET BIL PRO GLU UBG Hb Table 1 Dipstick Result Table (Red Bull

Effect on Solute Concentration in Dialysis Tubing After 30 Minutes - AP Biology - Lab Report

1662 words - 7 pages , and additional help from my teacher. Previous to the lab, my biology class had recently covered diffusion, and how it functions within the cell. We first covered basic diffusion: the movement of a substance from high concentrations to low concentrations. Then we moved onto the cell semipermeable membrane is what dictates which substances pass through. Following that, we covered osmosis: the movement of a solvent into a higher concentration in

The Effects of Smoking on Lung Tissue - Biology - Lab Report

2559 words - 11 pages slides of both normal lung and bronchogenic carcinoma were also observed and drawn to assist with the study. After completing the experiment, the mean beat frequency was calculated for each variable using both trials from each student in the lab. A bar graph was then created using these averages to display the data. The standard error was also calculated for each average and plotted onto each bar on the graph. ResultsD. Perklic The data graphed in

Effect of Temperature on Enzyme Activity - AP biology - Research Lab

898 words - 4 pages The Effect of Temperature on Enzyme Activity The purpose of this lab practice is to investigate the effects of temperature on the denaturation of enzymes. Introduction: The main biological concept explored was enzyme activity, enzymes are biological molecules (proteins) that act as catalysts and help complex reactions occur everywhere in life. Enzymes are impacted by temperature and pH of their environment, because these factors can denature an

Biology IA Idea, Toothpaste Experiment - Grade 12 - Internal Assessment

559 words - 3 pages temperature, the agar medium will set like stiff gelatin. 8. Sterilize the inoculating loop by passing it through the bunsen burner. 9. Make a solution of each toothpaste by taking 1g of toothpaste in 2 ml distilled water. 10. Open a sterile agar gel plate near the bunsen burner. The flame will kill the bacteria in the air. 11. By using an inoculating loop gently collect bacteria from your mouth and place it on the surface of all agar plates, 12

Chapter 12: Mendel’s Experiments and Heredity - Bio 142 - Biology

733 words - 3 pages General Biology 142 Chapter 12 Part 2 SQP Mendel’s Experiments and Heredity Section 12.2 Characteristics and Traits Question 1: What is incomplete dominance? Provide an example. Response: when the heterozygous phenotype appears to be intermediate Incomplete Dominance in Four O’clock Flowers 1:2:1 Phenotypic Ratio produced when crossing 2 pink plants Question 2: What are multiple alleles? Provide an example. Response: usually there are more

Biology AT1-Osmosis In Potatoes

1956 words - 8 pages Biology AT1 - OsmosisSkill area PAimTo investigate a factor which might affect the movement of water in and out of cells.IntroductionOsmosis is the movement of water in and out of cell membranes, which act as semi-permeable membranes. Semi permeable membranes are cells, which only take in a limited amount of a liquid. The water passes from a region of high to a region of low concentration, until the two are equal in concentrations of water.If a

The Birds and The Beaks - Darwin's Finches - Grade 11 Biology - Assignment

966 words - 4 pages environment and allow them to survive. This eventually became important evidence to the theory of evolution. The purpose of this lab is to mimic Darwin’s theory of evolution/survival of the fittest by pretending to be birds with different beaks to see the possible advantages and disadvantages certain beaks may have over others. My hypothesis is that there will be a difference in which bird can pick up the most food/which one flourish and evolve. If

My Most Influential Discovery in Biology - Biology - essay

594 words - 3 pages My Most Influential Discovery in Biology The biggest discovery in the biology field that influenced my life is Antonie Van Leeuwenhoek’s discovery of the microscope lens. I feel like this helped all of humanity because with that discovery came so much power over disease and illness.This idea first appeared in 1632 but was modified for along time to make it more efficient.Today they are used in every lab and doctor office i know of. Without them

Science Biology Exam Study For Grade 11 Students - dddd - dddd

640 words - 3 pages mirror and converging/diverging lens · Understand rules of refraction · Use n=c/v to calculate the index of refraction and the speed of light in a certain medium · Understand phenomena of refraction and total internal reflection · Use the lens equation and the magnification equation to determine the variables (you will be given the equations) Biology · Understand the cell theory : cell is simplest unit of life, all cells come from pre-existing