MATERIALS:MicroscopeOnionIodine Stain3 ToothpicksCheekMethylene Blue StainTomatoWaterPotatoCover SlipSlidePURPOSE:The purpose of this lab is to explore different cells under a microscope. To experience what different cells look like and to locate/describe the cells inside the tomato, potato, cheek, and onion.PROCEDURE:Onion Epidermis CellObtain a very thin piece of skin from the inside layer of an onionMount it on a slide, using a small drop of iodine stain, and a cover slip.Observe under low and high powerTake note of the shape of the cells, appearance of the nucleus, location of the nucleus, and location/shape of any vacuoles that may be presentDraw the cells under high power: label the cell wall, nucleus, cytoplasm, and any vacuolesHuman Epithelial CellsGently scrape the inside of your cheek with a toothpickSmear the material on the slide add a drop of methylene blue stain, and a cover slipObserve under low and high powerNote the shape, size, and nucleolusDraw the cells under high power label the plasma membrane, cytoplasm, nucleus, and nucleolusTomato Pulp CellsUsing a toothpick, smear a small amount of pulp on a slide add a drop of water and cover slipObserve under low and high powerTake note of the size, shape, and color of the cellsDraw the cells under high power; label cell wall, cytoplasm, nucleus, and chloroplasts.Potato CellsScrape a few cells from a potato with a toothpick and place on a slide using a cover slipObserve under low powerAdd a drop of iodine stain to the side of the cover slipObserve under high powerNote the structures that turn blue-blackDraw the cells under high power; label the cell wall, cytoplasm, and starch granulesDATA: CONCLUSION:5
